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天然药物功能蛋白质组学学科组
职  务: 中国科学院昆明动物研究所副所长
学  历: 博士
电  话: +86 871 65197578
传  真: +86 871 65199086
电子邮件: rlai@mail.kiz.ac.cn
通讯地址: 云南省昆明市教场东路32号 中国科学院昆明动物研究所    650223
其他主页:
  简  历

研究员,1972年出生,国家杰出青年基金获得者;中国科学院昆明动物研究所研究员,博士生导师 

主要学历

1991.9-1995.7 西南师范大学,生物学,学士

1995.9-2001.7 中科院昆明动物研究所,动物学,博士

主要科研工作经历

2001.8-2002.3,中国科学院昆明动物研究所,助理研究员

2002.4-2004.6 利物浦大学,博士后

2004.7-今,中国科学院昆明动物研究所,副所长,研究员

到目前以第一或者通讯作者身份共发表SCI论文200余篇,如Nature ImmunologyImmunityNature CommunicationsChemical ReviewsProc Natl Acad SciMol Cell ProteomicsHypertensionJ Med ChemFASEB JAllergyFree Radic Biol MedJBC等;受邀担任J Venom Res副主编;申请发明专利70多项

  研究方向

科技成就

长期致力于生物生存策略及环境适应机制研究、探索有毒动物(如蜈蚣、牛虻等)捕食和防御策略,揭示生物环境适应的物质基础与分子机制、并基于天然活性物质进行新药研发

1)研究高原两栖动物环境适应机制,从臭蛙皮肤发现第三套抗氧化系统,对皮肤保护、衰老研究与药物研究应用具重要意义(Mol Cell Proteomics 20098:571-583),该工作得到德国哥廷根大学Burkhard Poeggeler教授高度赞赏这是该领域的先驱工作,近期内没有任何其他工作在同领域有类似影响; 

2)研究两栖动物微生物防御机制,从铃蟾等识别了超过400种抗菌肽,占目前世界两栖动物抗菌肽总数40%以上,揭示两栖动物环境适应机制,也为抗感染药物研发提供源头资源(Chem Rev. 2015;115:1760-846),并利用抗菌肽作为工具研究动脉粥样硬化疾病机理(Immunity 2015;43:1137-47); 

3)研究树蛙对捕食者的防御策略,从华西树蛙发现神经毒素,这是首次发现的两栖动物基因编码的神经毒(J Biol Chem. 2009284:22079-86),为镇痛新药研发提供了候选药物、并被美国生物化学与分子生物学联合会作为亮点工作重点报道及被Science Daily等二十多家国际媒体报道; 

4)研究吸血节肢动物吸血策略,揭示牛虻从其宿主成功吸血分子机制,从牛虻唾液腺发现>100种抗血栓和抗感染多肽,为药物研发提供源头资源(Mol Cell Proteomics. 2008;7:582-90.Mol Cell Proteomics. 2009;8:2071-9);探索埃及伊蚊与宿主互作机制及吸血策略,发现其唾液腺成分通过抑制宿主免疫而协助寨卡病毒传播(Nature Immunology, 19(4):342-353),并为自身免疫药物研发提供及源头资源; 

5)研究蜈蚣捕食策略,首次发现钠通道NaV1.7专一抑制剂(PNAS 2013;110:17534-9),被NatureNature Medicine进行专门评论,并被推为Faculty 1000,认为是目前全世界发现的最好的治疗慢性疼痛候选药物;蜈蚣毒素成分SsTx阻断离子通道KCNQ从而实现对巨大猎物高效捕食(PNAS 2018115:1646-1651),该工作被Science、华盛顿邮报、德国新闻社等专门评论;蜈蚣通过其毒素成分激活辣椒素受体诱导捕食者产生剧烈疼痛,从而逃避捕食(Nat Commun. 2015;6:8297),为镇痛药物研发提供新思路和新的工具药物; 

通过这些工作,揭示有毒动物环境适应策略;以通讯作者身份在Immunity IF 25)、Nature ImmunologyIF 22, PNAS(2)Nature Communications, Chemical ReviewsIF 50)等著名国际刊物上发表SCI研究论文170篇(具体论文情况可通过如下链接查询:https://www.ncbi.nlm.nih.gov/pubmed/?term=lai+ren),识别了自主知识产权活性多肽1300多种,提供候选药物资源,获发明专利授权60项,奠定了我们在该领域的国际领先地位,受邀在国际顶级刊物Chemical ReviewsIF 50)发表长篇综述论文(Chem Rev. 2015;115:1760-846)、利用毒素多肽研发药物或工具药物(如一工具药物已在全球销售RHT-3832-PIhttps://www.pepnet.com/Products/Detail/3766/Rh-Tx);利用有毒动物资源推动山区农村脱贫致富,十一五、十二五期连续两次被云南省评为扶贫工作先进集体。

  承担科研项目

目前承担国家自然科学基金重点项目、国家杰出青年基金、科技部973、重大新药创制专项、以及中国科学院方向性项目。 

  专家类别
享受政府特殊津贴专家;国家杰出青年基金获得者;研究员
  社会任职
  获奖及荣誉

1.  国家杰出青年基金获得者,2010

2.  基于生物生存策略的有毒动物中药功能成分定向挖掘技术体系,2013年,国务院,国家技术发明奖,二等,第一完成人

3.  两栖动物活性多肽的结构与功能研究,2008年,云南省政府,云南省自然科学奖,一等,第一完成人

4.  吸血蜱与其宿主相互作用的分子机制研究,2009年,云南省政府,云南省自然科学奖,二等,第一完成人

5.  全国创新争先奖状,2017年,中国科协、科技部、人力资源和社会保障部、国有资产监督管理委员会,个人奖

6.  中医药管理局突出贡献奖,2017年,中医药管理局,个人奖

7.  中国青年科技奖,2011年,中国科协,个人奖

8.  中国科学院王宽诚西部学者突出贡献奖,2010年,中国科学院,个人奖

9.  第十届中国科学院十大杰出青年2010年,中国科学院,个人奖

10. 谈家桢生命科学创新奖,2010年,科技部、上海市生物医药行业协会,个人奖

11.    万人计划科技领军人才,科技部

12.    连续2年(20162017)指导的博士研究生获得中科院百篇博优秀论文

13.    云南省“十二五”十大科技人物

14.    云南省“天然活性多肽研究与利用”重点实验室主任

15.    云南省“天然活性多肽”工程实验室主任

  代表论著

2018

1. Wang G, Long C, Liu W, Xu C, Zhang M, Li Q, Lu Q, Meng P, Li D, Rong M, Sun Z, Luo X, Lai R. Novel Sodium Channel Inhibitor From Leeches. Front Pharmacol. 2018 Mar 6;9:186.

 

2. Jin L, Guo X, Shen C, Hao X, Sun P, Li P, Xu T, Hu C, Rose O, Zhou H, Yang M, Qin CF, Guo J, Peng H, Zhu M, Cheng G, Qi X, Lai R. Salivary factor LTRIN from Aedes aegypti facilitates the transmission of Zika virus by interfering with the lymphotoxin-β receptor. Nat Immunol. 2018 Apr;19(4):342-353.

 

3. Zeng X, Li P, Chen B, Huang J, Lai R, Liu J, Rong M. Selective Closed-State Nav1.7 Blocker JZTX-34 Exhibits Analgesic Effects against Pain. Toxins (Basel). 2018 Feb 2;10(2). pii: E64.

 

4. Tu Q, Hao J, Zhou X, Yan L, Dai H, Sun B, Yang D, An S, Lv L, Jiao B, Chen C, Lai R, Shi P, Zhao X. CDKN2B deletion is essential for pancreatic cancer development instead of unmeaningful co-deletion due to juxtaposition to CDKN2A.

Oncogene. 2018 Jan 4;37(1):128-138.

 

5. Luo L, Li B, Wang S, Wu F, Wang X, Liang P, Ombati R, Chen J, Lu X, Cui J, Lu Q, Zhang L, Zhou M, Tian C, Yang S, Lai R. Centipedes subdue giant prey by blocking KCNQ channels. Proc Natl Acad Sci U S A. 2018 Feb 13;115(7):1646-1651.

 

6. Li Y, Guo X, Hu C, Du Y, Guo C, Di Wang, Zhao W, Huang G, Li C, Lu Q, Lai R, Xu T, Qi X. Type I IFN operates pyroptosis and necroptosis during multidrug-resistant A. baumannii infection. Cell Death Differ. 2018 Jan 19. doi: 10.1038/s41418-017-0041-z.

 

2017

 

1. Aluthwattha ST, Harrison RD, Ranawana KB, Xu C, Lai R, Chen J. Does spatial variation in predation pressure modulate selection for aposematism? Ecol Evol. 2017 Aug 15;7(18):7560-7572.

 

2. Yang S, Yang F, Zhang B, Lee BH, Li B, Luo L, Zheng J, Lai R. A bimodal activation mechanism underlies scorpion toxin-induced pain. Sci Adv. 2017 Aug 2;3(8):e1700810.

 

3. Jin L, Fang M, Chen M, Zhou C, Ombati R, Hakim MA, Mo G, Lai R, Yan X, Wang Y, Yang S. An insecticidal toxin from Nephila clavata spider venom. Amino Acids. 2017 Jul;49(7):1237-1245.

 

4. Fang YQ, Shen CB, Luan N, Yao HM, Long CB, Lai R, Yan XW. In vivo antimalarial activity of synthetic hepcidin against Plasmodium berghei in mice. Chin J Nat Med. 2017 Mar;15(3):161-167.

 

5. Luan N, Zhou C, Li P, Ombati R, Yan X, Mo G, Rong M, Lai R, Duan Z, Zheng R.

Joannsin, a novel Kunitz-type FXa inhibitor from the venom of Prospirobolus joannsi. Thromb Haemost. 2017 Jun 2;117(6):1031-1039.

 

2016

1. Meng P, Huang H, Wang G, Yang S, Lu Q, Liu J, Lai R, Rong M. A Novel Toxin from Haplopelma lividum Selectively Inhibits the NaV1.8 Channel and Possesses Potent Analgesic Efficacy. Toxins (Basel). 2016 Dec 26;9(1). pii: E7.

 

2. He X, Shen C, Lu Q, Li J, Wei Y, He L, Bai R, Zheng J, Luan N, Zhang Z, Rong M, Lai R. Prokineticin 2 Plays a Pivotal Role in Psoriasis. EBioMedicine. 2016 Nov;13:248-261.

 

3. Luan N, Shen W, Liu J, Wen B, Lin Z, Yang S, Lai R, Liu S, Rong M. A Combinational Strategy upon RNA Sequencing and Peptidomics Unravels a Set of Novel Toxin Peptides in Scorpion Mesobuthus martensii. Toxins (Basel). 2016 Oct 5;8(10).

 

4. Liu WH, Chen Y, Bai XW, Yao HM, Zhang XG, Yan XW, Lai R. Identification and characterization of a novel neuropeptide (neuropeptide Y-HS) from leech salivary gland of Haemadipsa sylvestris. Chin J Nat Med. 2016 Sep;14(9):677-682.

 

5. Yao HM, Wang G, Liu YP, Rong MQ, Shen CB, Yan XW, Luo XD, Lai R. Phenolic acids isolated from the fungus Schizophyllum commune exert analgesic activity by inhibiting voltage-gated sodium channels. Chin J Nat Med. 2016 Sep;14(9):661-670.

 

6. Ma H, Xiao-Peng T, Yang SL, Lu QM, Lai R. Protease inhibitor in scorpion (Mesobuthus eupeus) venom prolongs the biological activities of the crude venom. Chin J Nat Med. 2016 Aug;14(8):607-14.

 

7. Lai Y, Li B, Liu W, Wang G, Du C, Ombati R, Lai R, Long C, Li H. Purification and Characterization of a Novel Kazal-Type Trypsin Inhibitor from the Leech of Hirudinaria manillensis. Toxins (Basel). 2016 Jul 23;8(8).

 

8. Rong M, Liu J, Zhang M, Wang G, Zhao G, Wang G, Zhang Y, Hu K, Lai R. A sodium channel inhibitor ISTX-I with a novel structure provides a new hint at the evolutionary link between two toxin folds. Sci Rep. 2016 Jul 13;6:29691.

 

9. Jin L, Bai X, Luan N, Yao H, Zhang Z, Liu W, Chen Y, Yan X, Rong M, Lai R, Lu Q. A Designed Tryptophan- and Lysine/Arginine-Rich Antimicrobial Peptide with Therapeutic Potential for Clinical Antibiotic-Resistant Candida albicans Vaginitis. J Med Chem. 2016 Mar 10;59(5):1791-9.

 

10. Kang D, Li B, Luo L, Jiang W, Lu Q, Rong M, Lai R. Curcumin shows excellent therapeutic effect on psoriasis in mouse model. Biochimie. 2016 Apr;123:73-80.

 

11. Wei NN, Lv HN, Wu Y, Yang SL, Sun XY, Lai R, Jiang Y, Wang K. Selective Activation of Nociceptor TRPV1 Channel and Reversal of Inflammatory Pain in Mice by a Novel Coumarin Derivative Muralatin L from Murraya alata. J Biol Chem. 2016 Jan 8;291(2):640-51.

 

12. Hao X, Tang X, Luo L, Wang Y, Lai R, Lu Q. A novel ranacyclin-like peptide with anti-platelet activity identified from skin secretions of the frog Amolops loloensis. Gene. 2016 Jan 15;576(1 Pt 1):171-5.

 

2015

 

1. Zhang Z, Meng P, Han Y, Shen C, Li B, Hakim MA, Zhang X, Lu Q, Rong M, Lai R. Mitochondrial DNA-LL-37 Complex Promotes Atherosclerosis by Escaping from Autophagic Recognition. Immunity. 2015 ;43(6):1137-47. 

 

2. Hakim MA, Yang S, Lai R. Centipede Venoms and Their Components: Resources for Potential Therapeutic Applications. Toxins (Basel). 2015 Nov 17;7(11):4832-51.

 

3. Hao X, Tang X, Luo L, Wang Y, Lai R, Lu Q. A novel ranacyclin-like peptide with anti-platelet activity identified from skin secretions of the frog Amolops loloensis. Gene. 2016 Jan 15;576(1 Pt 1):171-5. 

 

4. Yang S, Yang F, Wei N, Hong J, Li B, Luo L, Rong M, Yarov-Yarovoy V, Zheng J, Wang K, Lai R. A pain-inducing centipede toxin targets the heat activation machinery of nociceptor TRPV1. Nat Commun. 2015 Sep 30;6:8297..

 

5. Hakim MA, Jiang W, Luo L, Li B, Yang S, Song Y, Lai R. Scorpion Toxin, BmP01, Induces Pain by Targeting TRPV1 Channel. Toxins (Basel). 2015 Sep 14;7(9):3671-87. 

 

6. Fang Y, Long C, Bai X, Liu W, Rong M, Lai R, An S. Two new types of allergens from the cockroach, Periplaneta americana. Allergy. 2015 70(12):1674-8.

 

7. Bai X, Yao H, Du C, Chen Y, Lai R, Rong M. An immunoregulatory peptide from tsetse fly salivary glands of Glossina morsitans morsitans. Biochimie. 2015 Nov;118:123-8. 

 

8. Wang YW, Tan JM, Du CW, Luan N, Yan XW, Lai R, Lu QM. A Novel Trypsin Inhibitor-Like Cysteine-Rich Peptide from the Frog Lepidobatrachus laevis Containing Proteinase-Inhibiting Activity. Nat Prod Bioprospect. 2015 Aug;5(4):209-14. 

 

9. Hu K, Jiang Y, Xie Y, Liu H, Liu R, Zhao Z, Lai R, Yang L. Small-Anion Selective Transmembrane "Holes" Induced by an Antimicrobial Peptide Too Short to Span Membranes. J Phys Chem B. 2015 Jul 9;119(27):8553-60. 

 

10. Zhong S, Wu J, Cui Y, Li R, Zhu S, Rong M, Lu Q, Lai R. Vascular endothelial growth factor from Trimeresurus jerdonii venom specifically binds to VEGFR-2.Biochimie. 2015 Sep;116:1-7. 

 

11. Jiang WB, Hakim M, Luo L, Li BW, Yang SL, Song YZ, Lai R, Lu QM. Purification and characterization of cholecystokinin from the skin of salamander Tylototriton verrucosus. Dongwuxue Yanjiu. 2015 May 18;36(3):174-7.

 

12. Xu X, Lai R. The chemistry and biological activities of peptides from amphibian skin secretions. Chem Rev. 2015 Feb 25;115(4):1760-846. 

 

13. Rong M, Yang S, Wen B, Mo G, Kang D, Liu J, Lin Z, Jiang W, Li B, Du C, Yang S, Jiang H, Feng Q, Xu X, Wang J, Lai R. Peptidomics combined with cDNA library unravel the diversity of centipede venom. J Proteomics. 2015 Jan 30;114:28-37.

 

14. Tang J, Fang Y, Han Y, Bai X, Yan X, Zhang Y, Lai R, Zhang Z. YY-39, a tick anti-thrombosis peptide containing RGD domain. Peptides. 2015 Jun;68:99-104. 

 

2014

 

1. Zhu Y, Li Z, Liu H, He X, Zhang Y, Jin J, Che J, Li C, Chen W, Lai R, Liu J. Novel analgesic peptides from the tree frog of Hyla japonica. Biochimie. 2014, 99:38-43.

 

 2. Zhu Y, Duan Z, Mo G, Shen C, Lv L, Chen W, Lai R. A novel 26RFa peptide containing both analgesic and anti-inflammatory functions from Chinese tree shrew. Biochimie. 2014, 102:112-6.

 

3. Zhang K, Ding W, Sun J, Zhang B, Lu F, Lai R, Zou Y, Yedid G. Antioxidant and antitumor activities of 4-arylcoumarins and 4-aryl-3,4-dihydrocoumarins. Biochimie. 2014 Dec;107 Pt B:203-10

 

4. Mu L, Tang J, Liu H, Shen C, Rong M, Zhang Z, Lai R. A potential wound-healing-promoting peptide from salamander skin. FASEB J. 2014Sep;28(9):3919-29.

 

5. Liu H, Duan Z, Tang J, Lv Q, Rong M, Lai R. A short peptide from frog skin accelerates diabetic wound healing. FEBS J. 2014281(20):4633-43. 

 

 6. Zhang Z, Gao L, Shen C, Rong M, Yan X, Lai R. A potent anti-thrombosis peptide(vasotab TY) from horsefly salivary glands. Int J Biochem Cell Biol. 201454:83-8.

 

7. Liu H, Mu L, Tang J, Shen C, Gao C, Rong M, Zhang Z, Liu J, Wu X, Yu H, Lai R. A potential wound healing-promoting peptide from frog skin. Int J Biochem Cell Biol. 2014, 49:32-41.

 

8. Zhong Y, Song B, Mo G, Yuan M, Li H, Wang P, Yuan M, Lu Q. A Novel Neurotoxin from Venom of the Spider, Brachypelma albopilosum. PLoS One. 2014, 9(10):e110221. 

 

9. Tang J, Liu H, Gao C, Mu L, Yang S, Rong M, Zhang Z, Liu J, Ding Q, Lai R. A small peptide with potential ability to promote wound healing. PLoS One. 2014, 9(3):e92082. 

 

10. Tang J, Fang Y, Han Y, Bai X, Yan X, Zhang Y, Lai R, Zhang Z. YY-39, a tick anti-thrombosis peptide containing RGD domain. Peptides. 2015 Jun;68:99-104.

 

2013

 

1. Zhu Y, Li Z, Liu H, He X, Zhang Y, Jin J, Che J, Li C, Chen W, Lai R, Liu J. Novel analgesic peptides from the tree frog of Hyla japonica. Biochimie.2014 Apr;99:38-43..

 

2. Yang S, Xiao Y, Kang D, Liu J, Li Y, Undheim EA, Klint JK, Rong M, Lai R, King GF.Discovery of a selective NaV1.7 inhibitor from centipede venom with analgesic efficacy exceeding morphine in rodent pain models. Proc Natl Acad Sci U S A. 2013;110(43):17534-9.

 

3. Liu YP, Lai R, Yao YG, Zhang ZK, Pu ET, Cai XH, Luo XD.Induced furoeudesmanes: a defense mechanism against stress in Laggera pterodonta, a Chinese herbal plant. Org Lett. 2013 4;15(19):4940-3.

 

4. Zhang Z, Mu L, Tang J, Duan Z, Wang F, Wei L, Rong M, Lai R. A small peptide with therapeutic potential for inflammatory acne vulgaris. PLoS One. 2013; 8(8):e72923.

 

5. Yan H, Liu Y, Tang J, Mo G, Song Y, Yan X, Wei L, Lai R. A novel antimicrobial peptide from skin secretions of the tree frog Theloderma kwangsiensis. Zoolog Sci. 2013;30(9):704-9.

 

6. Liu R, Zhang Z, Liu H, Hou P, Lang J, Wang S, Yan H, Li P, Huang Z, Wu H, Rong M, Huang J, Wang H, Lv L, Qiu M, Ding J, Lai R.Human β-defensin 2 is a novel opener of Ca2+-activated potassium channels and induces vasodilation and hypotension in monkeys. Hypertension. 2013; 62(2):415-25.

 

7. Liu H, Zhao B, Chen Y, You D, Liu R, Rong M, Ji W, Zheng P, Lai R. Multiple coagulation factor deficiency protein 2 contains the ability to support stem cell self-renewal.FASEB J. 2013; 27(8):3298-305.

 

8. Song Y, Ji S, Liu W, Yu X, Meng Q, Lai R. Different expression profiles of bioactive peptides in Pelophylax nigromaculatus from distinct regions. Biosci Biotechnol Biochem.2013;77 (5):1075-9.

 

9. Wei L, Wu J, Liu H, Yang H, Rong M, Li D, Zhang P, Han J, Lai R.A mycobacteriophage-derived trehalose-6,6'-dimycolate-binding peptide containing both antimycobacterial and anti-inflammatory abilities. FASEB J. 2013; 27(8):3067-77.

 

10. Wei L, Yang J, He X, Mo G, Hong J, Yan X, Lin D, Lai R. Structure and function of a potent lipopolysaccharide-binding antimicrobial and anti-inflammatory peptide. J Med Chem. 2013; 56(9):3546-56.

 

11. An S, Chen L, Long C, Liu X, Xu X, Lu X, Rong M, Liu Z, Lai R. Dermatophagoides farinae allergens diversity identification by proteomics. Mol Cell Proteomics. 2013;12(7):1818-28.

 

12. Fan Y, Huang ZY, Cao CC, Chen CS, Chen YX, Fan DD, He J, Hou HL, Hu L, Hu XT, Jiang XT, Lai R, Lang YS, Liang B, Liao SG, Mu D, Ma YY, Niu YY, Sun XQ, Xia JQ, Xiao J, Xiong ZQ, Xu L, Yang L, Zhang Y, Zhao W, Zhao XD, Zheng YT, Zhou JM, Zhu YB, Zhang GJ, Wang J, Yao YG.Genome of the Chinese tree shrew. Nat Commun. 2013; 4:1426. 

 

13. Liu D, Wang Y, Wei L, Ye H, Liu H, Wang L, Liu R, Li D, Lai R.Snake venom-like waprin from the frog of Ceratophrys calcarata contains antimicrobial function. Gene. 2013;  514(2) :99-104.

 

14. Wang G, Wang Y, Ma D, Liu H, Li J, Zhang K, Yang X, Lai R, Liu J.Five novel antimicrobial peptides from the Kuhl's wart frog skin secretions, Limnonectes kuhlii. Mol Biol Rep. 2013; 40(2):1097-102.

 

15. He X, Yang S, Wei L, Liu R, Lai R, Rong M. Antimicrobial peptide diversity in the skin of the torrent frog, Amolops jingdongensis.Amino Acids. 2013; 44(2):481-7.

 

2012

 

1. Meng P, Wei L, Yang S, Liu H, Liu R, Lai R. A novel frog skin peptide containing function to induce muscle relaxation. Biochimie. Biochimie. 2012. 94(12): 2508-13.

 

2. Wang Y, Yan H, Wang Y, Yang H, Wei L, Xiao Y, Ye H, Lai R, Liu R. Proteomics and transcriptome analysis coupled with pharmacological test reveals the diversity of anti-thrombosis proteins from the medicinal insect, Eupolyphaga sinensis. Insect Biochem Mol Biol. 2012, 42(8):537-44. 

 

3. Yang S, Liu Z, Xiao Y, Li Y, Rong M, Liang S, Zhang Z, Yu H, King GF, Lai R. Chemical punch packed in venoms makes centipedes excellent predators. Mol Cell Proteomics. 2012;11(9):640-50

 

4. Yan H, Wei L, He X, Liu H, Yang S, Lai R, Rao D. A novel myotropic peptide from the skin secretions of the tree frog, Polypedates pingbianensis. Biochimie. 2012, 94(8):1718-23.

 

5. An S, Chen L, Wei JF, Yang X, Ma D, Xu X, Xu X, He S, Lu J, Lai R. Purification and characterization of two new allergens from the venom of Vespa magnifica. PLoS One. 2012; 7(2):e31920.

 

6. Xu X, Francischetti IM, Lai R, Ribeiro JM, Andersen JF. Structure of protein having inhibitory disintegrin and leukotriene scavenging functions contained in single domain. J Biol Chem. 2012, 287(14):10967-76. 

 

7. Yan X, Zhong J, Liu H, Liu C, Zhang K, Lai R. The cathelicidin-like peptide derived from panda genome is a potential antimicrobial peptide. Gene. 2012, 25;492(2):368-74. 

 

8. Wu H, Li J, Zhang Q, Yan X, Guo L, Gao X, Qiu M, Jiang X, Lai R, Chen H. A novel small Odorranalectin-bearing cubosomes: preparation, brain delivery and pharmacodynamic study on amyloid-β2 5-3 5-treated rats following intranasal administration. Eur J Pharm Biopharm. 2012, 80(2):368-78. 

 

9. Hao X, Yang H, Wei L, Yang S, Zhu W, Ma D, Yu H, Lai R. Amphibian cathelicidin fills the evolutionary gap of cathelicidin in vertebrate. Amino Acids. 2012, 43(2):677-85.

 

10. Yan X, Liu H, Yang X, Che Q, Liu R, Yang H, Liu X, You D, Wang A, Li J, Lai R. Bi-functional peptides with both trypsin-inhibitory and antimicrobial activities are frequent defensive molecules in Ranidae amphibian skins. Amino Acids. 2012, 43(1):309-16. 

 

2011

 

1. Wang Y, Zhang Z, Chen L, Guang H, Li Z, Yang H, Li J, You D, Yu H, Lai R. Cathelicidin-BF, a snake cathelicidin-derived antimicrobial peptide, could be an excellent therapeutic agent for acne vulgaris. PLoS One. 2011, 6(7):e22120. 

 

2. Wu J, Liu H, Yang H, Yu H, You D, Ma Y, Ye H, Lai R. Proteomic analysis of skin defensive factors of tree frog Hyla simplex. J Proteome Res. 2011, 10(9):4230-40.

 

3. Liu R, Mu L, Liu H, Wei L, Yan T, Chen M, Zhang K, Li J, You D, Lai R. Two antimicrobial and nematicidal peptides derived from sequences encoded Picea sitchensis. J Pept Sci. 2011, 17(9):627-31. 

 

4. Yang H, Wang Y, Xiao Y, Wang Y, Wu J, Liu C, Ye H, Li F, Yu H, Lai R. A bi-functional anti- thrombosis protein containing both direct-acting fibrin(ogen)olytic and plasminogen-activating activities. PLoS One. 2011, 6(3):e17519. 

 

5. Ma D, Xu X, An S, Liu H, Yang X, Andersen JF, Wang Y, Tokumasu F, Ribeiro JM, Francischetti IM, Lai R. A novel family of RGD-containing disintegrins (Tablysin-15) from the salivary gland of the horsefly Tabanus yao targets αIIbβ3 or αVβ3 and inhibits platelet aggregation and angiogenesis. Thromb Haemost. 2011, 105(6):1032-45.

 

6. Wei L, Dong L, Zhao T, You D, Liu R, Liu H, Yang H, Lai R. Analgesic and anti-inflammatory effects of the amphibian neurotoxin, anntoxin. Biochimie. 2011, 93(6):995-1000.

 

7. Wen Z, Yan Z, Hu K, Pang Z, Cheng X, Guo L, Zhang Q, Jiang X, Fang L, Lai R. Odorranalectin- conjugated nanoparticles: preparation, brain delivery and pharmacodynamic study on Parkinson's disease following intranasal administration. J Control Release. 2011, 151(2):131-8. 

 

8. Ma D, Li Y, Dong J, An S, Wang Y, Liu C, Yang X, Yang H, Xu X, Lin D, Lai R. Purification and characterization of two new allergens from the salivary glands of the horsefly, Tabanus yao. Allergy. 2011; 66: 101-9. 

 

9. An S, Ma D, Wei JF, Yang X, Yang HW, Yang H, Xu X, He S, Lai R. A novel allergen Tab y1 with inhibitory activity of platelet aggregation from salivary glands of horseflies. Allergy. 2011, 66(11): 1420-7.

 

10. Liu R, Liu H, Ma Y, Wu J, Yang H, Ye H, Lai R. There are abundant antimicrobial peptides in brains of two kinds of Bombina toads. J Proteome Res. 2011, 10(4):1806-15. 

 

11. Liu X, Liu R, Wei L, Yang H, Zhang K, Liu J, Lai R. Two novel antimicrobial peptides from skin secretions of the frog, Rana nigrovittata. J Pept Sci. 2011, 17(1):68-72.

 

2010

 

1. Wu J, Wang Y, Liu H, Yang H, Ma D, Li J, Li D, Lai R. Two immunoregulatory peptides with antioxidant activity from tick salivary glands J Biol Chem. 2010; 285: 16606-13.

 

2. Liu C, Hong J, Yang H, Wu J, Ma D, Li D, Lin D, Lai R. Frog skins keep redox homeostasis by antioxidant peptides with rapid radical-scavenging ability. Free Radic Biol Med. 2010; 48: 1173-81.

 

3. Chen M, Che Q, Wang X, Li J, Yang H, Li D, Zhang K, Lai R. Cloning and characterization of the first amphibian bradykinin gene. Biochimie. 2010; 92: 226-31. 

 

4. Lai R. Combined peptidomics and genomics approach to the isolation of amphibian antimicrobial peptides. Methods Mol Biol. 2010; 615: 177-90.

 

5. Wang M, Wang Y, Wang A, Song Y, Ma D, Yang H, Ma Y, Lai R. Five novel antimicrobial peptides from skin secretions of the frog, Amolops loloensis. Comp Biochem Physiol B. 2010;155: 72-6. 

 

6. Ma Y, Liu C, Liu X, Wu J, Yang H, Wang Y, Li J, Yu H, Lai R. Peptidomics and genomics analysis of novel antimicrobial peptides from the frog, Rana nigrovittata. Genomics. 2010; 95: 66-71. 

 

7. Ma D, Gao L, An S, Song Y, Wu J, Xu X, Lai R. A horsefly saliva antigen 5-like protein containing RTS motif is an angiogenesis inhibitor. Toxicon. 2010; 55: 45-51. 

 

8. Lu Z, Wang Y, Zhai L, Che Q, Wang H, Du S, Wang D, Feng F, Liu J, Lai R, Yu H. Novel cathelicidin-derived antimicrobial peptides from Equus asinus. FEBS J. 2010; 277: 2329-39. 

 

2009

 

1. Wang WX, Wang YP, Deng XJ, Dang XL, Tian JH, Yi HY, Li YF, He XF, Cao Y, Xia QY, Lai R, Wen SY, Paskowitz S. Molecular and functional characterization of a c-type lysozyme from the Asian corn borer, Ostrinia furnacalis. J Insect Sci. 2009; 9: 17. 

 

2. Zhang CX, Yang SY, Xu MX, Sun J, Liu H, Liu JR, Liu H, Kan F, Sun J, Lai R, Zhang KY. Serratia nematodiphila sp. nov., associated symbiotically with the entomopathogenic nematode Heterorhabditidoides chongmingensis (Rhabditida: Rhabditidae). Int J Syst Evol Microbiol. 2009; 59: 1603-8. 

 

3. Song Y, Lu Y, Wang L, Yang H, Zhang K, Lai R. Purification, characterization and cloning of two novel tigerinin-like peptides from skin secretions of Fejervarya cancrivora. Peptides. 2009; 30: 1228-32.

 

4. You D, Hong J, Rong M, Yu H, Liang S, Ma Y, Yang H, Wu J, Lin D, Lai R. The first gene-encoded amphibian neurotoxin. J Biol Chem. 2009; 284: 22079-86. 

 

5. Ma D, Wang Y, Yang H, Wu J, An S, Gao L, Xu X, Lai R. Anti-thrombosis repertoire of blood-feeding horsefly salivary glands. Mol Cell Proteomics. 2009; 8: 2071-9.

 

6. Liu H, Zhou Y, Liu R, Zhang KY, Lai R. Bacillus solisalsi sp. nov., a halotolerant, alkaliphilic bacterium isolated from soil around a salt lake. Int J Syst Evol Microbiol. 2009; 59: 1460-4.

 

7. Yang SY, Liu H, Liu R, Zhang KY, Lai R. Saccharibacillus kuerlensis sp. nov., isolated from a desert soil. Int J Syst Evol Microbiol. 2009; 59: 953-7. 

 

8. Zhou Y, Wei W, Wang X, Xu L, Lai R. Azospirillum palatum sp. nov., isolated from forest soil in Zhejiang province, China. J Gen Appl Microbiol. 2009; 55: 1-7. 

 

9. Zhou Y, Wei W, Wang X, Lai R. Proposal of Sinomonas flava gen. nov., sp. nov., and description of Sinomonas atrocyanea comb. nov. to accommodate Arthrobacter atrocyaneus. Int J Syst Evol Microbiol. 2009; 59: 259-63.

 

10. Yang H, Wang X, Liu X, Wu J, Liu C, Gong W, Zhao Z, Hong J, Lin D, Wang Y, Lai R. Antioxidant peptidomics reveals novel skin antioxidant system. Mol Cell Proteomics. 2009; 8: 571-83. 

 

2008

 

1. Zhou Y, Wei W, Che Q, Xu Y, Wang X, Huang X, Lai R. Bacillus pallidus sp. nov., isolated from forest soil. Int J Syst Evol Microbiol. 2008; 58: 2850-4. 

 

2. Wang Y, Hong J, Liu X, Yang H, Liu R, Wu J, Wang A, Lin D, Lai R. Snake cathelicidin from Bungarus fasciatus is a potent peptide antibiotics. PLoS One. 2008; 3: e3217. 

 

3. Wei W, Zhou Y, Wang X, Huang X, Lai R. Sphingobacterium anhuiense sp. nov., isolated from forest soil. Int J Syst Evol Microbiol. 2008; 58: 2098-101. 

 

4. Li J, Wu H, Hong J, Xu X, Yang H, Wu B, Wang Y, Zhu J, Lai R, Jiang X, Lin D, Prescott MC, Rees HH. Odorranalectin is a small peptide lectin with potential for drug delivery and targeting. PLoS One. 2008; 3: e2381. 

 

5. Liu R, Liu H, Zhang CX, Yang SY, Liu XH, Zhang KY, Lai R. Sphingobacterium siyangense sp. nov., isolated from farm soil. Int J Syst Evol Microbiol. 2008; 58: 1458-62. 

 

6. Liu R, Liu H, Feng H, Wang X, Zhang CX, Zhang KY, Lai R. Pseudomonas duriflava sp. nov., isolated from a desert soil. Int J Syst Evol Microbiol. 2008; 58: 1404-8. 

 

7. Yan X, Feng H, Yu H, Yang X, Liu J, Lai R. An immunoregulatory peptide from salivary glands of the horsefly, Hybomitra atriperoides. Dev Comp Immunol. 2008; 32: 1242-7. 

 

8. Li J, Wu J, Wang Y, Xu X, Liu T, Lai R, Zhu H. A small trypsin inhibitor from the frog of Odorrana grahami. Biochimie. 2008; 90: 1356-61.

 

9. Zhang C, Liu J, Xu M, Sun J, Yang S, An X, Gao G, Lin M, Lai R, He Z, Wu Y, Zhang K. Heterorhabditidoides chongmingensis gen. nov., sp. nov. (Rhabditida: Rhabditidae), a novel member of the entomopathogenic nematodes. J Invertebr Pathol. 2008; 98: 153-68. 

 

10. Liu H, Liu R, Yang SY, Gao WK, Zhang CX, Zhang KY, Lai R Flavobacterium anhuiense sp. nov., isolated from field soil. Int J Syst Evol Microbiol. 2008; 58: 756-60. 

 

11. Liu SB, He YY, Zhang Y, Lee WH, Qian JQ, Lai R, Jin Y. A novel non-lens betagamma-crystallin and trefoil factor complex from amphibian skin and its functional implications. PLoS One. 2008; 3: e1770.

 

12. Wang A, Wang J, Hong J, Feng H, Yang H, Yu X, Ma Y, Lai R. A novel family of antimicrobial peptides from the skin of Amolops loloensis. Biochimie. 2008; 90: 863-7.

 

13. Han J, You D, Xu X, Han W, Lu Y, Lai R, Meng Q. An anticoagulant serine protease from the wasp venom of Vespa magnifica. Toxicon. 2008; 51: 914-22. 

 

14. Che Q, Zhou Y, Yang H, Li J, Xu X, Lai R. A novel antimicrobial peptide from amphibian skin secretions of Odorrana grahami. Peptides. 2008; 29: 529-35. 

 

15. Lu X, Ma Y, Wu J, Lai R. Two serine protease inhibitors from the skin secretions of the toad, Bombina microdeladigitora. Comp Biochem Physiol B 2008; 149: 608-12. 

 

16. Zhou Y, Zhang YQ, Zhi XY, Wang X, Dong J, Chen Y, Lai R, Li WJ. Description of Sinobacter flavus gen. nov., sp. nov., and proposal of Sinobacteraceae fam. nov. Int J Syst Evol Microbiol. 2008; 58: 184-9.

 

17. Lu J, Yang H, Yu H, Gao W, Lai R, Liu J, Liang X. A novel serine protease inhibitor from Bungarus fasciatus venom. Peptides. 2008; 29: 369-74. 

 

18. Zhang K, Liu H, Sun J, Liu J, Fei K, Zhang C, Xu M, Sun J, Ma X, Lai R, Wu Y, Lin M. Molecular Phylogeny of Geographical Isolates of Bursaphelenchus xylophilus: Implications on the Origin and Spread of this Species in China and Worldwide. J Nematol. 2008; 40: 127-37. 

 

2007

 

1. Wang X, Song Y, Li J, Liu H, Xu X, Lai R, Zhang K. A new family of antimicrobial peptides from skin secretions of Rana pleuraden. Peptides. 2007; 28: 2069-74. 

 

2. Yu H, Wang X, Liu J, Lai R. A novel algesic peptide derived from skin secretions of the frog Amolops loloensis. J Pept Sci. 2007; 13: 798-802.

 

3. Chen L, Li Y, Li J, Xu X, Lai R, Zou Q. An antimicrobial peptide with antimicrobial activity against Helicobacter pylori. Peptides. 2007; 28: 1527-31. 

 

4. Liu X, Wang Y, Cheng L, Song Y, Lai R. Isolation and cDNA cloning of cholecystokinin from the skin of Rana nigrovittata. Peptides. 2007; 28: 1540-4.

 

5. Zhou Y, Dong J, Wang X, Huang X, Zhang KY, Zhang YQ, Guo YF, Lai R, Li WJ. Chryseobacterium flavum sp. nov., isolated from polluted soil. Int J Syst Evol Microbiol. 2007; 57: 1765-9. 

 

6. Yu H, Yang H, Ma D, Lv Y, Liu T, Zhang K, Lai R, Liu J. Vespid chemotactic peptide precursor from the wasp, Vespa magnifica (Smith). Toxicon. 2007; 50: 377-82. 

 

7. Li J, Zhang C, Xu X, Wang J, Yu H, Lai R, Gong W. Trypsin inhibitory loop is an excellent lead structure to design serine protease inhibitors and antimicrobial peptides. FASEB J. 2007; 21: 2466-73. 

 

8. Xu C, Ma D, Yu H, Li Z, Liang J, Lin G, Zhang Y, Lai R. A bactericidal homodimeric phospholipases A2 from Bungarus fasciatus venom. Peptides. 2007; 28: 969-73. 

 

 9. Li J, Xu X, Xu C, Zhou W, Zhang K, Yu H, Zhang Y, Zheng Y, Rees HH, Lai R, Yang D, Wu J. Anti-infection peptidomics of amphibian skin. Mol Cell Proteomics. 2007; 6: 882-94. 

 

 10. Zhou Y, Wang X, Liu H, Zhang KY, Zhang YQ, Lai R, Li WJ. Pontibacter akesuensis sp. nov., isolated from a desert soil in China. Int J Syst Evol Microbiol. 2007; 57: 321-5. 

 

 11. Li J, Yu H, Xu X, Wang X, Liu D, Lai R. Multiple bombesin-like peptides with opposite functions from skin of Odorrana grahami. Genomics. 2007; 89: 413-8. 

 

2006

 

1. Xu X, Yang H, Yu H, Li J, Lai R. The mastoparanogen from wasp. Peptides. 2006; 27: 3053-7. 

 

2. Li J, Liu T, Xu X, Wang X, Wu M, Yang H, Lai R. Amphibian tachykinin precursor. Biochem Biophys Res Commun. 2006; 350: 983-6.

 

3. Lu Y, Li J, Yu H, Xu X, Liang J, Tian Y, Ma D, Lin G, Huang G, Lai R. Two families of antimicrobial peptides with multiple functions from skin of rufous-spotted torrent frog, Amolops loloensis. Peptides. 2006; 27: 3085-91. 

 

4. Zhou Z, Yang H, Xu X, Wang X, Lai R. The first report of kininogen from invertebrates. Biochem Biophys Res Commun. 2006; 347: 1099-102. 

 

5. Liang J, Han Y, Li J, Xu X, Rees HH, Lai R. A novel bradykinin-like peptide from skin secretions of rufous-spotted torrent frog, Amolops loloensis. Peptides. 2006; 27: 2683-7.

 

6. Yu D, Liang J, Yu H, Wu H, Xu C, Liu J, Lai R. A tick B-cell inhibitory protein from salivary glands of the hard tick, Hyalomma asiaticum asiaticum. Biochem Biophys Res Commun. 2006; 343: 585-90. 

 

7. Xu X, Li J, Han Y, Yang H, Liang J, Lu Q, Lai R. Two antimicrobial peptides from skin secretions of Rana grahami. Toxicon. 2006; 47: 459-64. 

 

8. Zhang YQ, Li WJ, Zhang KY, Tian XP, Jiang Y, Xu LH, Jiang CL, Lai R. Massilia dura sp. nov., Massilia albidiflava sp. nov., Massilia plicata sp. nov. and Massilia lutea sp. nov., isolated from soils in China. Int J Syst Evol Microbiol. 2006; 56: 459-63. 

 

9. Xu X, Li J, Lu Q, Yang H, Zhang Y, Lai R. Two families of antimicrobial peptides from wasp (Vespa magnifica) venom. Toxicon. 2006; 47: 249-53. 

 

10. Li J, Xu X, Yu H, Yang H, Huang Z, Lai R Direct antimicrobial activities of PR-bombesin. Life Sci. 2006; 78: 1953-6.

 

11. Yu D, Sheng Z, Xu X, Li J, Yang H, Liu Z, Rees HH, Lai R. A novel antimicrobial peptide from salivary glands of the hard tick, Ixodes sinensis. Peptides. 2006; 27: 31-5. 

 

2005

 

1. Zhang YX, Lai R, Lee WH, Zhang Y. Frog albumin is expressed in skin and characterized as a novel potent trypsin inhibitor. Protein Sci. 2005; 14: 2469-77.

 

2. Lee WH, Zhang J, Zhang YX, Jin Y, Lai R, Zhang Y. Maximin 9, a novel free thiol containing antimicrobial peptide with antimycoplasma activity from frog Bombina maxima. FEBS Lett. 2005; 579: 4443-8.

 

3. Lee WH, Liu SB, Shen JH, Jin Y, Lai R, Zhang Y. Identification and molecular cloning of a novel neuromedin U analog from the skin secretions of toad Bombina maxima. Regul Pept. 2005; 129: 43-7. 

 

4. Lee WH, Li Y, Lai R, Li S, Zhang Y, Wang W. Variety of antimicrobial peptides in the Bombina maxima toad and evidence of their rapid diversification. Eur J Immunol. 2005;35: 1220-9. 

 

5. Liang JG, Zhang J, Lai R, Rees HH. An opioid peptide from synganglia of the tick, Amblyomma testindinarium. Peptides. 2005; 26: 603-6.

 

2004

 

1. Lai R, Takeuchi H, Jonczy J, Rees HH, Turner PC. A thrombin inhibitor from the ixodid tick, Amblyomma hebraeum. Gene. 2004; 342: 243-9.

 

2. Lai R, Takeuchi H, Lomas LO, Jonczy J, Rigden DJ, Rees HH, Turner PC. A new type of antimicrobial protein with multiple histidines from the hard tick, Amblyomma hebraeum. FASEB J. 2004; 18: 1447-9. 

 

3. Lai R, Lomas LO, Jonczy J, Turner PC, Rees HH. Two novel non-cationic defensin-like antimicrobial peptides from haemolymph of the female tick, Amblyomma hebraeum. Biochem J. 2004; 379: 681-5.

 

2003

 

1. Lai R, Liu H, Lee WH, Zhang Y. Bombinakinin M gene associated peptide, a novel bioactive peptide from skin secretions of the toad Bombina maxima. Peptides. 2003; 24: 199-204.

 

2. Lai R, Liu H, Lee WH, Zhang Y. Two novel Bv8-like peptides from skin secretions of the toad Bombina maxima. Comp Biochem Physiol B 2003; 134: 509-14. 

 

2002

 

 1. Lai R, Yang DM, Lee WH, Zhang Y. Biological activities of skin secretions of the salamander Tylototriton verrucosus. J Nat Toxins. 2002; 11: 245-50. 

 

 2. Lai R, Liu H, Hui Lee W, Zhang Y. An anionic antimicrobial peptide from toad Bombina maxima. Biochem Biophys Res Commun. 2002; 295: 796-9. 

 

 3. Lai R, Liu H, Lee WH, Zhang Y. A novel proline rich bombesin-related peptide (PR-bombesin) from toad Bombina maxima. Peptides. 2002; 23: 437-42. 

 

 4. Lai R, Zheng YT, Shen JH, Liu GJ, Liu H, Lee WH, Tang SZ, Zhang Y. Antimicrobial peptides from skin secretions of Chinese red belly toad Bombina maxima. Peptides. 2002; 23: 427-35.

 

5. Lai R, Liu H, Lee WH, Zhang Y. Identification and cloning of a trypsin inhibitor from skin secretions of Chinese red-belly toad Bombina maxima. Comp Biochem Physiol B 2002; 131: 47-53. 

 

2001

 

 1. Lai R, Liu H, Hui Lee W, Zhang Y. A novel bradykinin-related peptide from skin secretions of toad Bombina maxima and its precursor containing six identical copies of the final product. Biochem Biophys Res Commun. 2001, 286: 259-63.

  研究团队

工作人员:

靳林 博士 副研究员 jinlin@ mail.kiz.ac.cn

吕秋敏 博士 副研究员 lvqm@mail.kiz.ac.cn

杨仕隆 博士 副研究员 yslzoology@163.com

张治业 博士 副研究员 zhangzhiye5225@163.com

李东升 高级工程师 lids@mail.kiz.ac.cn

段自磊 博士 助理研究员 duanzileikiz@126.com

申传斌 博士 助理研究员 lvqm@mail.kiz.ac.cn

郝雪 博士 助理研究员 snowwhite_0001@163.com

王淦 博士 助理研究员 wanggan@mail.kiz.ac.cn

孟平 博士 博士后 mengping@mail.kiz.ac.cn

研究生:

唐小芃,李博文,罗雷,王云飞,韩亚蓝,陆先翠,张旻,王鑫,陈雪,殷一珠,张兴龙,龙爱琳,张浩,RoseJamesZhanserik

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