遗传资源与进化国家重点实验室     首页 > 研究队伍
郑 萍  研究员
哺乳动物胚胎发育研究组学科组
职  务:
学  历: 博士
电  话: +86 871 68125422
传  真:
电子邮件: zhengp@mail.kiz.ac.cn
通讯地址: 云南省昆明市教场东路32号 中国科学院昆明动物研究所    650223
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  简  历
博士,研究员,以猕猴和小鼠为模型,长期从事哺乳动物卵细胞发生及着床前胚胎发育的研究。2009年5月进入昆明动物研究所,加入遗传资源与进化国家重点实验室,并组建哺乳动物胚胎发育课题组。研究方向为配子发育生物学及着床前胚胎发育调控。

1990.09-1994.07,重庆师范学院生物系获学士学位。
1995.09 - 2001.08,中国科学院昆明动物研究所,获博士学位。
2001.08 - 2003.03,中国科学院昆明动物研究所助理研究员。
2003.03 - 2005.06,美国Fels Institute for Cancer Research and Molecular Biology, Temple University School of Medicine博士后。
2005.06 – 2009.03,美国国立卫生研究院(NIH)博士后。
2009.06 – 至今,中国科学院昆明动物研究所研究员,组建哺乳动物胚胎发育课题组,担任课题组长。

 

  研究方向

  1. 多能干细胞维持遗传物质稳定性的机制。多能干细胞可分化为机体的所有细胞类型,在再生医学中具有巨大的应用前景。其遗传物质的稳定性维持是多能干细胞应用于再生医学的重要前提。DNA损伤反应(DNA damage response)是细胞应对内源及外源DNA损伤,维持遗传物质稳定性的重要机制。我们在小鼠及人胚胎干细胞中鉴定参与DNA损伤反应的重要分子,并深入研究作用机制。

  2.   雌性生殖细胞的再生研究。哺乳动物雌性配子的发生是否存在再生机制一直是生殖生物学领域长期争议的话题。虽然有少数研究报道了小鼠及人卵巢生殖干细胞的分离和体外培养,但没有确凿的证据支持生理状况下存在卵巢生殖干细胞及其再生功能。我们以小鼠为模型,通过遗传手段着重研究生理状态下是否存在卵巢生殖干细胞的再生活动,并在此基础上研究卵巢生殖干细胞的分子生物学特性。

  3. 猕猴卵细胞和早期胚胎发育的分子调控。由于伦理原因,人类自身的发育过程一直都非常不清楚,特别是早期胚胎的发育调控。利用云南的灵长类资源,我们开展了猕猴从卵细胞到着床前胚胎发育的系统研究,重点探讨最早期的细胞命运分化机制。

 

 
  承担科研项目

1.中国科学院干细胞先导专项猕猴早期胚胎发育调控研究,资助年限2011.01-2015.12. 

2.自然科学基金面上项目“Filia 维持胚胎干细胞染色体稳定性的作用及机制研究,资助年限2012.01-2015.12. 

3.国家重点基础研究发展计划非编码RNA与干细胞命运调控子课题,资助年限2011.01-2015.12. 

4.国家重点基础研究发展计划生殖病理和毒理的灵长类模型的建立和研究子课题,资助年限2012.01-2016.12. 

5.云南省应用基础研究计划重点项目“ECAT1维持人多能干细胞遗传物质稳定性的功能及机制研究”,资助年限 2013.10-2016.09. 

  专家类别
研究员
  社会任职
  获奖及荣誉
  代表论著

1. Guo K, Li CH, Wang XY, He DJ, Zheng P#. Germ stem cells are active in postnatal mouse ovary under physiological conditions. Mol Hum Reprod, 2016, 22(5): 316-328 (该工作受到杂志同期特邀评论 Mol Hum Reprod, 2016, 22(5):313-315). 

2.Lu YQ, He XC, Zheng P#. Decrease in expression of maternal effect gene Mater is associated with maternal ageing in mice. Mol Hum Reprod, 2016, 22(4): 252-260. 

3.Zhao B, Zhang WD, Duan YL, Lu YQ, Cun YX, Li CH, Guo K, Nie WH, Li L, Zhang R, Zheng P#. Filia is an ESC-specific regulator of DNA damage response and safeguards genomic stability. Cell Stem Cell, 2015, 16: 684-698. 

4.Zhang W, Xiao MS, Ji S, Tang J, Xu L, Li X, Li M, Wang HZ, Jiang HY, Zhang DF, Wang J, Zhang S, Xu XF, Yu L, Zheng P, Chen X, Yao YG. Promoter variant rs2301228 on the neural cell adhesion molecule 1 gene confers risk of schizophrenia in Han Chinese. Schizophr Res, 2014, 160(1-3):88-96. 

5.Yu XJ, Yi Z, Gao Z, Qin D, Zhai Y, Chen X, Ou-Yang Y, Wang ZB, Zheng P, Zhu MS, Wang H, Sun QY, Dean J, Li L. The subcortical maternal complex controls symmetric division of mouse zygotes by regulating F-actin dynamics. Nat Commun, 2014, 5:4887. 

6.Tan T, Zhang Y, Ji W#, Zheng P#. miRNA Signature in Mouse Spermatogonial Stem Cells Revealed by High-Throughput Sequencing. Biomed Res Int, 2014, 154251. 

7.Zhao YQ, Ji S, Wang JK, Huang JF#, ZhengP#. mRNA-Seq and microRNA-Seq whole-transcriptome analysis of  rhesus monkey ESC  neural differentiation  revealed the potential regulators of rosette neural stem cells. DNA Research, 2014, 21(5):541-54. 

8. Zhang XO, Yin QF, Wang HB, Zhang Y, Chen T, Zheng P, Lu X, Chen LL, Yang L. Species-specific alternative splicing leads to unique expression of sno-lncRNAs. BMC Genomics, 2014, 15: 287. 

9.Liu H, Zhao B, Chen Y, You D, Liu R, Rong M, Ji W, Zheng P, Lai R. Multiple coagulation factor deficiency protein 2 contains the ability to support stem cell self-renewal. FASEB J, 2013, 27(8):3298-305. 

10.Zheng P#, Baibakov B, Wang XH, Dean J#. PtdIns(3,4,5)P3 is constitutively synthesized and required for spindle translocation during meiosis in mouse oocytes. J Cell Sci, 2013, 126(Pt 3):715-21. 

11.Li L, Zheng P, Dean J. Maternal control of early mouse development. Development, 2010, 137: 859-870. 

12.Zheng P, Dean J. Role of Filia, a Maternal Effect Gene, in Maintaining Euploidy during Cleavage Stage Mouse Embryogenesis. Proc Natl Acad Sci USA, 2009, 106(18):7473-8. 

13.Ohsugi M, Zheng P, Baibakov B, Li L, Dean J. Maternally derived FILIA-MATER complex localizes asymmetrically in cleavage-stage mouse embryos. Development, 2008, 135(2):259-269. 

14.Zheng P, Vassena R, Latham KE. Effects of in vitro oocyte maturation and embryo culture on the expression of glucose transporters, glucose metabolism and insulin signaling genes in rhesus monkey oocytes and preimplantation embryos. Mol Hum Reprod, 2007, 13: 361-371. 

15.Zheng P, Dean J. Oocyte-specific genes affect folliculogenesis, fertilization, and early development (Invited review). Semin Reprod Med, 2007, 25(4):243-251. 

16.Zheng P. Effects of in vitro maturation of monkey oocytes on their developmental capacity (Invited review). Anim Reprod Sci, 2007, 98:56-71. 

17.Zheng P, Vassena R, latham K. Expression and downregulation of WNT signaling pathway genes in rhesus monkey oocytes and embryos. Mol Reprod Dev, 2006, 73: 667-677. 

18.Zheng P, Patel B, McMenamin M, Moran E, Paprocki AM, Kihara M, Schramm RD, Latham KE. Effects of follicle size and oocyte maturation conditions on maternal message RNA regulation and gene expression in rhesus monkey oocytes and embryos. Biol Reprod, 2005, 72:890-897.  

19.Zheng P, Schramm RD, Latham KE. Developmental regulation and in vitro culture effects on expression of DNA repair and cell cycle checkpoint control genes in rhesus monkey oocytes and embryos. Biol Reprod, 2005, 72: 1359-1369.   

20.Zheng P, Patel B, McMenamin M, Reddy, S, Paprocki AM, Schramm RD, Latham KE. The primate embryo gene expression resource:  A novel resource to facilitate rapid analysis of gene expression patterns in non-human primate oocytes and preimplantation stage embryos. Biol Reprod, 2004, 70: 1411-1418. 

21.Zheng P, Patel B, McMenamin M, Paprocki AM, Schramm RD, Nagl N, Wilsker D, Wang G, Moran E, Latham KE. Expression of genes encoding chromatin regulatory factors in developing rhesus monkey oocytes and preimplantation stage embryos: Possible roles in genome activation. Biol Reprod, 2004, 70: 1419-1427. 

22.Si W, Zheng P, Li YH, Dinnyes A, Ji WZ. Effect of Glycerol and Dimethyl Sulfoxide on cyropreservation of rhesus monkey (Macaca mulatta) sperm. Am J Primatol, 2004, 62: 301-306. 

23.Zheng P, Si W, Bavister BD, Yang JF, Ding CH, Ji WZ. 17-b Estradiol and progesterone improve in-vitro cytoplasmic maturation of oocytes from unstimulated prepubertal and adult rhesus monkeys. Hum Reprod, 2003, 18(10): 2137-2144. 

24.Zheng P, Bavistser BD, Ji WZ. Amino acid requirements for maturation of rhesus monkey oocytes in culture. Reproduction, 2002; 124, 515-525.  

25.Zheng P, Wang H, Bavister BD, Ji WZ. Maturation of rhesus monkey oocytes in chemically defined culture media and their functional assessmental by IVF and embryo development. Hum Repro,2001, 16(2): 300-305. 

26.Zheng P, Bavister BD, Ji WZ. Energy substrate requirement for in vitro maturation of oocytes from unstimulated adult rhesus monkeys. Mol Reprod Dev, 2001, 58: 348-355.  

27.Zheng P, Si W, Wang H, Zou RJ, Bavister BD, Ji WZ*. Effect of age and breeding season on the developmental capacity of oocytes from unstimulated and FSH-stimulated rhesus monkeys. Biol Reprod, 2001, 64: 1417-1421. 

28.Si W, Zheng P, Tang XH, He XC, Wang H, Bavister BD, Ji WZ. Cryopreservation of rhesus monkey (Macaca mulatta) spermatozoa and their functional assessment by in vitro fertilization. Cryobiology, 2000, 41(3): 232-240. 

  研究团队

工作人员
和协超 高级实验师

赵  博 助理研究员

张锦娟 研究实习员

陈忠良 研究实习员

在读研究生

李朝晖  2011
王鑫轶  2012
何大健  2012
郭  琨  2012
张伟道  2013
班文赞  2013
李竞争  2014
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