研究队伍
中科院院士
学科组负责人
百千万人才
杰出青年科学基金
优秀青年科学基金
万人计划
中青年科技创新领军人才
云南省高层人才
研究所青年人才
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研究队伍 -- 哺乳动物胚胎发育研究组
  郑萍    研究员
职  务:
学  历: 博士
电  话: +86 871 65197853
传  真:
电子邮件: zhengp@mail.kiz.ac.cn
通讯地址: 云南省昆明市教场东路32号 中国科学院昆明动物研究所    650223
其他主页:
 
  简  历

2009.06 至今,中国科学院昆明动物研究所研究员,组建哺乳动物胚胎发育课题组,担任课题组长。

2005.06 — 2009.03,美国国立卫生研究院(NIH)博士后。

2003.03 — 2005.06,美国Fels Institute for Cancer Research and Molecular Biology, Temple University School of Medicine博士后。

2001.08 — 2003.03,中国科学院昆明动物研究所助理研究员。

1995.09 — 2001.08,中国科学院昆明动物研究所,获博士学位。

1990.09 — 1994.07,重庆师范学院生物系获学士学位。

  研究方向

1. 干细胞维持基因组稳定性的特征和分子调控研究

  干细胞是机体发育和组织稳态维持的基础,其遗传物质的稳定性维持对于正常发育及组织器官的稳态维持至关重要。由于功能特殊,干细胞在遗传物质稳定性调控上有其独特的特征和机制。我们以胚胎干细胞和神经干细胞为对象,研究干细胞调控DNA损伤反应(DNA damage response)和DNA复制压力反应(replication stress response)的特殊分子机制。这些研究将有助于推动干细胞的安全应用,并有助于理解相关发育异常和疾病的致病机理。

2. 生殖干细胞的基础和应用研究

  雌性生殖细胞的再生研究:哺乳动物雌性配子的发生是否存在再生机制一直是生殖生物学领域长期争议的话题,目前尚没有确凿的证据支持生理状况下存在卵巢生殖干细胞及其再生功能。我们以小鼠为模型,通过遗传手段着重研究生理状态下是否存在卵巢生殖干细胞及其再生活动,并在此基础上研究卵巢生殖干细胞的生物学特性和再生活动调控。

  雄性生殖干细胞研究:树鼩(类灵长类动物)和猕猴(非人灵长类)与人的亲缘关系接近。我们重点研究树鼩和猕猴的精原干细胞的分子特征和体外长期扩增培养体系,并利用精原干细胞系进行基因修饰,创建疾病动物模型,模拟和研究人类疾病。

3. 灵长类早期胚胎发育

  由于伦理原因,人类自身的发育过程一直都非常不清楚,特别是早期胚胎的发育调控。利用云南的灵长类资源,我们开展了猕猴从卵细胞到着床前胚胎发育的系统研究,重点探讨最早期的细胞命运分化及发育多能性调控。

  承担科研项目

1. 国家自然科学基金-云南联合基金重点项目,U1702284, 阿尔茨海默症(AD)转基因树鼩模型的创建及有效性评价,2018/01-2021/12,主持

2. 国家自然科学基金面上项目,31571484,小鼠卵巢生殖干细胞分子标记的筛选及干细胞功能研究,2016/01-2019/12,主持

3. 国家重点研发计划, 代谢、自噬和DNA损伤修复协同维持多能干细胞干性和染色体稳定性的机理研究, 2016/10-2021/09,课题骨干

4. 国家重点研发计划,卵巢早衰病因学及临床防治研究,2017/10-2020/09, 课题骨干

5. 中国科学院战略性先导科技专项B类“动物复杂性状的进化解析与调控”, XDB130106002017/05-2019/06,课题骨干

6. 云南省高端科技人才计划,2015HA038,利用树鼩精原干细胞建立树鼩基因修饰技术,2015/01-2019/12,主持

  专家类别
研究员
  社会任职
 
  获奖及荣誉
 
  代表论著

1. Liu D#, Wang X#, He D#, Sun C#, He X, Yan L, Li Y, Han JJ*, Zheng P*. Single-cell RNA-sequencing reveals the existence of naive and primed pluripotency in pre-implantation rhesus monkey embryos. Genome Research, 2018, 28:1481–1493. (封面论文,期刊5年影响因子13.796)

 

2.    Zhao B#, Zhang WD#, Cun YX, Li JZ, Liu Y, Gao J, Zhu HW, Zhou H, Zhang RG, Zheng P*. Mouse embryonic stem cells have increased capacity for replication fork restart driven by the specific Filia-Floped protein complex. Cell Research, 2018, 28(1):69-89. (期刊5年影响因子15.973)

 

3.    Fan Y#, Luo R#, Su L-Y, Xiang Q, Yu D, Xu L, Chen J-Q, Bi R, Wu D-D, Zheng P*, Yao Y-G*. Does the genetic feature of the Chinese tree shrew (Tupaia belangeri chinensis) support its potential as a viable model for Alzheimer’s disease research? Journal of Alzheimers Disease, 2018; 61(3):1015-1028.

 

4.    Wang XY#, Liu DH#, He DJ, Suo SB, Xia X, He XC, Han JJ*, Zheng P*. Transcriptome analyses of rhesus monkey pre-implantation embryos reveal a reduced capacity for DNA double strand break (DSB) repair in primate oocytes and early embryos. Genome Research, 2017, 27(4):567-579.

 

5.    Li CH, Yan LZ, Ban WZ, Tu Q, Wu Y, Wang L, Bi R, Ji S, Ma YH, Nie WH, Lv LB, Yao YG*, Zhao XD*, Zheng P*. Long-term propagation of tree shrew spermatogonial stem cells in culture and successful generation of transgenic offspring. Cell Research, 2017, 27(2):241-252.

 

6.    Guo K#, Li CH#, Wang XY, He DJ, Zheng P*. Germ stem cells are active in postnatal mouse ovary under physiological conditions. Molecular Human Reproduction, 2016, 22(5): 316-328 (该工作受到杂志同期特邀评论, Mol Hum Reprod, 2016, 22:313-315).

 

7.    Lu YQ, He XC, Zheng P*. Decrease in expression of maternal effect gene Mater is associated with maternal ageing in mice. Molecular Human Reproduction, 2016, 22(4): 252-260.

 

8.    Zhao B, Zhang WD, Duan YL, Lu YQ, Cun YX, Li CH, Guo K, Nie WH, Li L, Zhang R, Zheng P*. Filia is an ESC-specific regulator of DNA damage response and safeguards genomic stability. Cell Stem Cell, 2015, 16: 684-698. (期刊5年影响因子24.565)

 

9.    Tan T#, Zhang Y#, Ji W*, Zheng P*. miRNA Signature in Mouse Spermatogonial Stem Cells Revealed by High-Throughput Sequencing. Biomed Res Int, 2014, 154251.

 

10. Zhao YQ#, Ji S#, Wang JK#, Huang JF*, Zheng P*. mRNA-Seq and microRNA-Seq whole-transcriptome analysis of rhesus monkey ESC neural differentiation revealed the potential regulators of rosette neural stem cells. DNA Research, 2014, 21(5):541-54.

 

11. Zheng P*, Baibakov B, Wang XH, Dean J*. PtdIns(3,4,5)P3 is constitutively synthesized and required for spindle translocation during meiosis in mouse oocytes. Journal of Cell Science, 2013, 126(Pt 3):715-21.

 

12. Li L, Zheng P, Dean J*. Maternal control of early mouse development. Development, 2010, 137: 859-870.

 

13. Zheng P, Dean J*. Role of Filia, a maternal effect gene, in maintaining euploidy during cleavage stage mouse embryogenesis. Proc Natl Acad Sci USA, 2009, 106(18):7473-8.

 

14. Ohsugi M, Zheng P, Baibakov B, Li L, Dean J*. Maternally derived FILIA-MATER complex localizes asymmetrically in cleavage-stage mouse embryos. Development, 2008, 135(2):259-269.

 

15. Zheng P, Vassena R, Latham KE*. Effects of in vitro oocyte maturation and embryo culture on the expression of glucose transporters, glucose metabolism and insulin signaling genes in rhesus monkey oocytes and preimplantation embryos. Molecular Human Reproduction, 2007, 13: 361-371.

 

16. Zheng P*, Dean J. Oocyte-specific genes affect folliculogenesis, fertilization, and early development (Invited review). Seminars in Reproductive Medicine, 2007, 25(4):243-251.

 

17. Zheng P*. Effects of in vitro maturation of monkey oocytes on their developmental capacity (Invited review). Animal Reproduction Science, 2007, 98:56-71.

 

18. Zheng P, Vassena R, Latham KE*. Expression and downregulation of WNT signaling pathway genes in rhesus monkey oocytes and embryos. Molecular Reproduction and Development, 2006, 73: 667-677.

 

19. Zheng P, Patel B, McMenamin M, Moran E, Paprocki AM, Kihara M, Schramm RD, Latham KE*. Effects of follicle size and oocyte maturation conditions on maternal message RNA regulation and gene expression in rhesus monkey oocytes and embryos. Biology of Reproduction, 2005, 72:890-897. 

 

20. Zheng P, Schramm RD, Latham KE*. Developmental regulation and in vitro culture effects on expression of DNA repair and cell cycle checkpoint control genes in rhesus monkey oocytes and embryos. Biology of Reproduction, 2005, 72: 1359-1369.  

 

21. Zheng P, Patel B, McMenamin M, Reddy, S, Paprocki AM, Schramm RD, Latham KE*. The primate embryo gene expression resource:  A novel resource to facilitate rapid analysis of gene expression patterns in non-human primate oocytes and preimplantation stage embryos. Biology of Reproduction, 2004, 70: 1411-1418.

 

22. Zheng P, Patel B, McMenamin M, Paprocki AM, Schramm RD, Nagl N, Wilsker D, Wang G, Moran E, Latham KE*. Expression of genes encoding chromatin regulatory factors in developing rhesus monkey oocytes and preimplantation stage embryos: Possible roles in genome activation. Biology of Reproduction, 2004, 70: 1419-1427.

 

23. Zheng P, Si W, Bavister BD, Yang JF, Ding CH, Ji WZ*. 17-b Estradiol and progesterone improve in-vitro cytoplasmic maturation of oocytes from unstimulated prepubertal and adult rhesus monkeys. Human Reproduction, 2003, 18(10): 2137-2144.

 

24. Zheng P, Bavistser BD, Ji WZ*. Amino acid requirements for maturation of rhesus monkey oocytes in culture. Reproduction, 2002; 124, 515-525. 

 

25. Zheng P, Wang H, Bavister BD, Ji WZ*. Maturation of rhesus monkey oocytes in chemically defined culture media and their functional assessmental by IVF and embryo development. Human Reproduction, 2001, 16(2): 300-305.

 

26. Zheng P, Bavister BD, Ji WZ*. Energy substrate requirement for in vitro maturation of oocytes from unstimulated adult rhesus monkeys. Molecular Reproduction and Development, 2001, 58: 348-355. 

 

27. Zheng P, Si W, Wang H, Zou RJ, Bavister BD, Ji WZ*. Effect of age and breeding season on the developmental capacity of oocytes from unstimulated and FSH-stimulated rhesus monkeys. Biology of Reproduction, 2001, 64: 1417-1421.

  研究团队

工作人员(3人):王林(助理研究员);郭琨(助理研究员);马怀孝(助理研究员)

研究生(12人):张伟道(博士研究生)、陈忠良(博士研究生)、李竞争(博士研究生)、孙春丽(博士研究生)、龚道华(博士研究生)、姜方洁(博士研究生)、李聪(硕士研究生)、李秀峰(硕士研究生)、宁雨琪(硕士研究生)、周漫漫(硕士研究生)、唐敏(硕士研究生)、谢恒(硕士研究生)