| 论文题目: | Trio Deep-sequencing does not Reveal Unexpected off-target and on-target Mutations in Cas9-edited Rhesus Monkeys |
| 作者: | Luo X, He Y, Zhang C, He X, Yan L, Li M, Hu T, Hu Y, Jiang J, Meng X, Ji W, Zhao X, Zheng P, Xu S, Su B |
| 联系作者: | sub@mail.kiz.ac.cn |
| 发表年度: | 2019 |
| DOI: | doi: 10.1038/s41467-019-13481-y |
| 摘要: | CRISPR-Cas9 is a widely-used genome editing tool, but its off-target effect and on-target complex mutations remain a concern, especially in view of future clinical applications. Non-human primates (NHPs) share close genetic and physiological similarities with humans, making them an ideal preclinical model for developing Cas9-based therapies. However, to our knowledge no comprehensive in vivo off-target and on-target assessment has been conducted in NHPs. Here, we perform whole genome trio sequencing of Cas9-treated rhesus monkeys. We only find a small number of de novo mutations that can be explained by expected spontaneous mutations, and no unexpected off-target mutations (OTMs) were detected. Furthermore, the long-read sequencing data does not detect large structural variants in the target region |
| 刊物名称: | Nature Communications |
| 论文出处: | https://www.nature.com/articles/s41467-019-13481-y |
| 影响因子: | 11.878(2018年) |
