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论文题目: BmILF and i-motif Structure are Involved in Transcriptional Regulation of BmPOUM2 in Bombyx mori
作者: Niu K, Zhang X, Deng H, Wu F, Ren Y, Xiang H, Zheng S, Liu L, Huang L, Zeng B, Li S, Xia Q, Song Q, Palli SR, Feng Q
联系作者: qlfeng@scnu.edu.cn
发表年度: 2017
DOI: doi:10.1093/nar/gkx1207
摘要:

Guanine-rich and cytosine-rich DNA can form four-stranded DNA secondary structures called G-quadruplex (G4) and i-motif, respectively. These structures widely exist in genomes and play important roles in transcription, replication, translation and protection of telomeres. In this study, G4 and i-motif structures were identified in the promoter of the transcription factor gene BmPOUM2, which regulates the expression of the wing disc cuticle protein gene (BmWCP4) during metamorphosis. Disruption of the i-motif structure by base mutation, anti-sense oligonucleotides (ASOs) or inhibitory ligands resulted in significant decrease in the activity of the BmPOUM2 promoter. A novel i-motif binding protein (BmILF) was identified by pull-down experiment. BmILF specifically bound to the i-motif and activated the transcription of BmPOUM2. The promoter activity of BmPOUM2 was enhanced when BmILF was over-expressed and decreased when BmILF was knocked-down by RNA interference. This study for the first time demonstrated that BmILF and the i-motifstructure participated in the regulation of gene transcription in insect metamorphosis and provides new insights into the molecular mechanism of the secondary structures in epigenetic regulation of gene transcription

刊物名称: Nucleic Acids Research
论文出处: https://academic.oup.com/journals/
影响因子: 10.162(2016年)