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论文题目: Direct Reprogramming of Fibroblasts via a Chemically Induced XEN-like State
作者: Li X, Liu D, Ma Y, Du X, Jing J, Wang L, Xie B, Sun D,Sun S, Jin X, Zhang X, Zhao T, Guan J, Yi Z, Lai W, Zheng P, Huang Z, Chang Y, Chai Z, Xu J, Deng H
联系作者: hongkui_deng@pku.edu.cn
发表年度: 2017
DOI: doi:10.1016/j.stem.2017.05.019
摘要:

Direct lineage reprogramming, including with small molecules, has emerged as a promising approach for generating desired cell types. We recently found that during chemical induction of induced pluripotent stem cells (iPSCs) from mouse fibroblasts, cells pass through an extra-embryonic endoderm (XEN)-like state. Here, we show that these chemically induced XEN-like cells can also be induced to directly reprogram into functional neurons, bypassing the pluripotent state. The induced neurons possess neuron-specific expression profiles, form functional synapses in culture, and further mature after transplantation into the adult mouse brain. Using similar principles, we were also able to induce hepatocyte-like cells from the XEN-like cells. Cells in the induced XEN-like state were readily expandable over at least 20 passages and retained genome stability and lineage specification potential. Our study therefore establishes a multifunctional route for chemical lineage reprogramming and may provide a platform for generating a diverse range of cell types via application of this expandable XEN-like state

刊物名称: Cell Stem Cell
论文出处: http://www.sciencedirect.com/science/article/pii/S1934590917301856
影响因子: 23.394(2016年)