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论文题目: Sequencing, de Novo Assembling and Annotating the Genome of the Endangered Chinese Crocodile Lizard Shinisaurus Crocodilurus
作者: Gao J, Li Q, Wang Z, Zhou Y, Martelli P, Li F, Xiong Z, Wang J, Yang H, Zhang G
联系作者: zhanggj@genomics.cn
外单位作者单位:
发表年度: 2017
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摘要:

The Chinese crocodile lizard, Shinisaurus crocodilurus, is the only living representative of the monotypic family Shinisauridae under the order Squamata. It is an obligate semi-aquatic, viviparous, diurnal species restricted to specific portions of mountainous locations in southwestern China and northeastern Vietnam. However, in the past several decades, this species has undergone a rapid decrease in population size due to illegal poaching and habitat disruption, making this unique reptile species endangered and listed in CITES Appendix II since 1990. A proposal to uplist it to Appendix I was passed at the CITES CoP17 in 2016. To promote the conservation of this species, we sequenced the genome of a male Chinese crocodile lizard using a whole-genome shotgun strategy on the Illumina HiSeq 2000 platform. In total, we generated ∼291Gb raw sequencing data (149X depth) from 13 libraries with insert sizes ranging from 250 bp to 40 kb. After filtering for PCR-duplicated and low-quality reads, ∼137 Gb clean data (70X depth) were obtained for genomeassembly. We yielded a draft genome assembly with a total length of 2.24 Gb and an N50 scaffold size of 1.47 Mb. The assembledgenome was predicted to contain 20 150 protein-coding genes and up to 1114 Mb (49.6%) of repetitive elements. The genomic resource of the Chinese crocodile lizard will contribute to deciphering the biology of this organism and provides an essential tool for conservation efforts. It also provides a valuable resource for future study of squamate evolution

刊物名称: Gigascience
论文出处: https://academic.oup.com/gigascience/article-lookup/doi/10.1093/gigascience/gix041
影响因子: 7.463(2015年)