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论文题目: |
Sequencing, de Novo Assembling and Annotating the Genome of the Endangered Chinese Crocodile Lizard Shinisaurus Crocodilurus |
作者: |
Gao J, Li Q, Wang Z, Zhou Y, Martelli P, Li F, Xiong Z, Wang J, Yang H, Zhang G |
联系作者: |
zhanggj@genomics.cn |
发表年度: |
2017 |
DOI: |
doi:10.1093/gigascience/gix041 |
摘要: |
The Chinese crocodile lizard, Shinisaurus crocodilurus, is the only living representative of the monotypic family Shinisauridae under the order Squamata. It is an obligate semi-aquatic, viviparous, diurnal species restricted to specific portions of mountainous locations in southwestern China and northeastern Vietnam. However, in the past several decades, this species has undergone a rapid decrease in population size due to illegal poaching and habitat disruption, making this unique reptile species endangered and listed in CITES Appendix II since 1990. A proposal to uplist it to Appendix I was passed at the CITES CoP17 in 2016. To promote the conservation of this species, we sequenced the genome of a male Chinese crocodile lizard using a whole-genome shotgun strategy on the Illumina HiSeq 2000 platform. In total, we generated ∼291Gb raw sequencing data (149X depth) from 13 libraries with insert sizes ranging from 250 bp to 40 kb. After filtering for PCR-duplicated and low-quality reads, ∼137 Gb clean data (70X depth) were obtained for genomeassembly. We yielded a draft genome assembly with a total length of 2.24 Gb and an N50 scaffold size of 1.47 Mb. The assembledgenome was predicted to contain 20 150 protein-coding genes and up to 1114 Mb (49.6%) of repetitive elements. The genomic resource of the Chinese crocodile lizard will contribute to deciphering the biology of this organism and provides an essential tool for conservation efforts. It also provides a valuable resource for future study of squamate evolution |
刊物名称: |
Gigascience |
论文出处: |
https://academic.oup.com/gigascience/article-lookup/doi/10.1093/gigascience/gix041 |
影响因子: |
7.463(2015年) |
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