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论文 |
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| 论文题目: |
A Robust Single Primate Neuroepithelial Cell Clonal Expansion System for Neural Tube Development and Disease Studies |
| 作者: |
Zhu X, Li B, Ai Z, Xiang Z, Zhang K, Qiu X, Chen Y, Li Y, Rizak JD, Niu Y, Hu X, Sun YE, Ji W, Li T |
| 联系作者: |
wji@kbimed.com; litq@kbimed.com |
| 发表年度: |
2016 |
| DOI: |
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| 摘要: |
Developing a model of primate neural tube (NT) development is important to promote many NT disorder studies in model organisms. Here, we report a robust and stable system to allow for clonal expansion of single monkey neuroepithelial stem cells (NESCs) to develop into miniature NT-like structures. Single NESCs can produce functional neurons in vitro, survive, and extensively regenerate neuron axons in monkey brain. NT formation and NESC maintenance depend on high metabolism activity and Wnt signaling. NESCs are regionally restricted to a telencephalic fate. Moreover, single NESCs can turn into radial glial progenitors (RGPCs). The transition is accurately regulated by Wnt signaling through regulation of Notch signaling and adhesion molecules. Finally, using the "NESC-TO-NTs'' system, we model the functions of folic acid (FA) on NT closure and demonstrate that FA can regulate multiple mechanisms to prevent NT defects. Our system is ideal for studying NT development and diseases. |
| 刊物名称: |
Stem Cell Reports |
| 论文出处: |
http://www.cell.com/stem-cell-reports/pdf/S2213-6711(15)00304-5.pdf |
| 影响因子: |
7.023 (2015年) |
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