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论文题目: Transcriptome analyses of rhesus monkey pre-implantation embryos reveal a reduced capacity for DNA double strand break repair in primate oocytes and early embryos
作者: Wang X, Liu D, He D, Suo S, Xia X, He X, Han JD, Zheng P.
联系作者: zhengp@mail.kiz.ac.cn.
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发表年度: 2017
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摘要: Pre-implantation embryogenesis encompasses several critical events including genome reprogramming, zygotic genome activation (ZGA), and cell fate commitment, most of which remain mechanistically unclear in primates. In addition, primates display a high rate of embryo wastage without any clear molecular basis. Understanding the factors involved in genome reprogramming and ZGA will help the generation of induced pluripotent stem cells with high efficiency. Moreover, explaining the molecular basis responsible for embryo wastage in primates will greatly expand our knowledge of species evolution. Here, we have carried out time-series RNA-seq studies in single and pooled rhesus monkey oocytes and pre-implantation embryos at representative developmental stages. By comparing to human and mouse data, we found that the transcriptome dynamics of monkey oocytes and embryos were very similar to those of human, but very different from those of mouse. We identified several classes of maternal and zygotic genes, whose expression peaks were highly correlated with the time frames of genome reprogramming, ZGA and cell fate commitment, respectively. Importantly, comparison of the ZGA-related network modules among the three species revealed a looser surveillance of genomic instability inprimate oocytes and embryos than in rodents, particularly in the pathways of DNA damage signaling and homology-directed DNAdouble strand break repair. This study highlights the importance of utilizing rhesus monkey to explore the molecular basis of genome reprogramming, ZGA, and genomic stability surveillance in human early embryogenesis, and provides important clues on how to improve the efficiency of homologous recombination-mediated gene editing in monkey transgenic technology.
刊物名称: Genome Research
论文出处: http://genome.cshlp.org/content/early/2017/02/21/gr.198044.115.full.pdf+html
影响因子: 11.351