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论文题目: Structure of a eukaryotic cyclic-nucleotide-gated channel
作者: Minghui Li,Xiaoyuan Zhou,Shu Wang,Ioannis Michailidis,Ye Gong, Deyuan Su, Huan Li, Xueming Li& Jian Yang
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发表年度: 2017
卷: 542
期: 7639
页码: 60-65
摘要: Cyclic-nucleotide-gated channels are essential for vision and olfaction. They belong to the voltage-gated ion channel superfamily but their activities are controlled by intracellular cyclic nucleotides instead of transmembrane voltage. Here we report a 3.5-Å-resolution single-particle electron cryo-microscopy structure of a cyclic-nucleotide-gated channel from Caenorhabditis elegans in the cyclic guanosine monophosphate (cGMP)-bound open state. The channel has an unusual voltage-sensor-like domain, accounting for its deficient voltage dependence. A carboxy-terminal linker connecting S6 and the cyclic-nucleotide-binding domain interacts directly with both the voltage-sensor-like domain and the pore domain, forming a gating ring that couples conformational changes triggered by cyclic nucleotide binding to the gate. The selectivity filter is lined by the carboxylate side chains of a functionally important glutamate and three rings of backbone carbonyls. This structure provides a new framework for understanding mechanisms of ion permeation, gating and channelopathy of cyclic-nucleotide-gated channels and cyclic nucleotide modulation of related channels.
刊物名称: Nature
论文出处: http://www.nature.com/nature/journal/vaop/ncurrent/pdf/nature20819.pdf
影响因子: 38.138(2015年)