| 论文题目: |
RNA sequencing shows no dosage compensation of the active X-chromosome |
| 作者: |
Yuanyan Xiong; Xiaoshu Chen; Zhidong Chen; Xunzhang Wang; Suhua Shi; Xueqin Wang; Jianzhi Zhang*; Xionglei He* |
| 联系作者: |
jianzhi@umich.edu; hexiongl@mail.sysu.edu.cn |
| 发表年度: |
2010 |
| DOI: |
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| 摘要: |
Mammalian cells from both sexes typically contain one active X chromosome but two sets of autosomes. It has previously been hypothesized that X-linked genes are expressed at twice the level of autosomal genes per active allele to balance the gene dose between the X chromosome and autosomes (termed 'Ohno's hypothesis'). This hypothesis was supported by the observation that microarray-based gene expression levels were indistinguishable between one X chromosome and two autosomes (the X to two autosomes ratio (X:AA) similar to 1). Here we show that RNA sequencing (RNA-Seq) is more sensitive than microarray and that RNA-Seq data reveal an X: AA ratio of similar to 0.5 in human and mouse. In Caenorhabditis elegans hermaphrodites, the X:AA ratio reduces progressively from similar to 1 in larvae to similar to 0.5 in adults. Proteomic data are consistent with the RNA-Seq results and further suggest the lack of X upregulation at the protein level. Together, our findings reject Ohno's hypothesis, necessitating a major revision of the current model of dosage compensation in the evolution of sex chromosomes. |
| 刊物名称: |
Nature Genetics |
| 论文出处: |
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| 影响因子: |
31.616 (2015) |
