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论文 |
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| 论文题目: |
Venomic and Transcriptomic Analysis of Centipede Scolopendra subspinipes dehaani |
| 作者: |
Zi-Chao Liu; Rong Zhang; Feng Zhao; Zhong-Ming Chen; Hao-Wen Liu; Yan-Jie Wang; Ping Jiang; Yong Zhang; Ying Wu; Jiu-Ping Ding*; Wen-Hui Lee*; Yun Zhang* |
| 联系作者: |
jpding@mail.hust.edu.cn; leewh@mail.kiz.ac.cn; zhangy@mail.kiz.ac.cn |
| 发表年度: |
2012 |
| DOI: |
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| 摘要: |
Centipedes have venom glands in their first pair of limbs, and their venoms contain a large number of components with different biochemical and pharmacological properties. However, information about the compositions and functions of their venoms is largely unknown. In this study, Scolopendra subspinipes dehaani venoms were systematically investigated by transcriptomic and proteomic analysis coupled with biological function assays. After random screening approximately 1500 independent clones, 1122 full length cDNA sequences, which encode 543 different proteins, were cloned from a constructed cDNA library using a pair of venom glands from a single centipede species. Neurotoxins, ion channel acting components and venom allergens were the main fractions of the crude venom as revealed by transcriptomic analysis. Meanwhile, 40 proteins/peptides were purified and characterized from crude venom of S. subspinipes dehaani. The N-terminal amino acid sequencing and mass spectrum results of 29 out of these 40 proteins or peptides matched well With their corresponding cDNAs. The purified proteins/peptides showed different pharmacological properties, including the following: (1) platelet aggregating activity; (2) anticoagulant activity; (3) phospholipase A(2) activity; (4) trypsin inhibiting activity; (5) voltage gated potassium channel activities; (6) voltage gated sodium channel activities; (7) voltage gated calcium channel activities. Most of them showed no significant similarity to other protein sequences deposited in the known public database. This work provides the largest number of protein or peptide candidates with medical-pharmaceutical significance and reveals the toxin nature of centipede S. subspinipes dehaani venom. |
| 刊物名称: |
Journal of Proteome Research |
| 论文出处: |
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| 影响因子: |
4.173 (2015) |
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