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论文 |
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| 论文题目: |
Characterization of Two Human Lung Adenocarcinoma Cell Lines by Reciprocal Chromosome Painting |
| 作者: |
Peng Kun-Jing; Wang Jin-Huan; Su Wei-Ting; Wang Xi-Cai; Yang Feng-Tang; Nie Wen-Hui* |
| 联系作者: |
whnie@mail.kiz.ac.cn |
| 发表年度: |
2010 |
| DOI: |
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| 摘要: |
Lung cancer is a leading cause of cancer death worldwide. Some lung cancer patients correlate with a gas of radon besides smoking. To search for common chromosomal aberrations in lung cancer cell lines established from patients induced by different factors, a combined approach of chromosome sorting, forward and reverse chromosome painting was used to characterize karyotypes of two lung adenocarcinoma cell lines: A549 and GLC-82 with the latter line derived from a patient who has suffered long-term exposure to environmental radon gas pollution. The chromosome painting results revealed that complex chromosomal rearrangements occurred in these two lung adenocarcinoma cell lines. Thirteen and twenty-four abnormal chromosomes were identified in A549 and GLC-82 cell lines, respectively. Almost half of abnormal chromosomes in these two cell lines were formed by non-reciprocal translocations, the others were derived from deletions and duplication/or amplification in some chromosomal regions. Furthermore, two apparently common breakpoints, HSA8q24 and 12q14 were found in these two lung cancer cell lines. |
| 刊物名称: |
Zoological Research |
| 论文出处: |
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